Mops buffer depc

Make up 2x 500ml and pH to 7 with 6M NaOH (DEPC). 20x SSC: 87.65g NaCl Mix enough 1x MOPS running buffer with sterile distilled water for gel tank. Add. 3.1 Treating water with DEPC to remove RNAse. 3.2 Denature RNA in Agarose DEPC. 10x MOPS buffer, made using DEPC-treated water. Dissolve 41.8g of MOPS, 6.8g of Sodium Acetate, 3.8g of Disodium EDTA in 800 ml DEPC water. 169 ml of 37% Formaldehyde, 100 ml of 10X MOPS, and 231 ml of DEPC water. 10 X Denaturing Gel Buffer and 10 X Gel Running Buffer.

Having a buffering range from 6.5-6.7, MOPS works well in formaldehyde gels at a concentration of 20nM. DEPC is added to ensure the buffer is nuclease. For standard medium-sized gel, dissolve 1.5 g agarose in 108 ml DEPC H2O. Pour running buffer (1X MOPS in DEPC H2O) in amount sufficient to cover gel.

Mix 167.45 g of MOPS, 16.4 g of NaOAc, 80 mL of 0.5M EDTA, pH to 7.0 with Add 5 mL 20X MOPS buffer, 92.5 mL DEPC-dH2O and microwave until melted. DNARNA electrophoresis-related reagents and buffer. 50X TAE Buffer (pH8.5) Weigh 41.8g MOPS, placed in 1L beaker, add about 800ml DEPC treated.

Northern Blot Protocol RNA Preparation Use DEPC

Ice-cold 100% and 80% ethanol solutions dedicated for RNA work. ? Loading dye for RNA samples. ? 10X MOPS buffer. ? Autoclaved DEPC-treated (DEPC.d). GOV (Daniel Solaiman) wrote: As far as MOPS buffers, don.t repeat my mistake Yes, you can DEPC-treat MOPS, but when you autoclave.

Cesar;s WMISH protocol

Formamide 15ul. 5?MOPS 6ul. formaldehyde 4.8ul. RNA 10ug ( less than 4.2ul). DEPC H2O add to 30ul. 65C 10min, put on ice, add 3ul 10?loading buffer. Prepared with DEPC for RNA applications. 10X concentrate contains 200 mM MOPS, 50 mM sodium acetate, 10 mM EDTA, pH 7.0 at 25°C. 10X MOPS electrophoresis buffer. 1, 41.8 g MOPS. 2, in 700 ml of sterile DEPCtreated H2O. 3, Adjust the pH to 7.0 with 2 N NaOH. 4, Add 20 ml of.

All water (MilliQ) for RNA experiments must be treated with DEPC (0.1% Place the gel with the comb in a gel box filled with 1 x MOPS buffer. 325MM MOPS pH7. 1M stock. 325µl. 162.5mM NaCl. 5M stock. 325µl. H2O. DEPC-treated 3.6ml. Hybridization: Wash the embryos with MOPS buffer, 3 times.

Melt 1.2g agarose in 87ml of DEPC water, by dispersing the agarose uniformly Bring the melted agarose to 60°C. Add 10ml 10X MOPS Buffer and 3ml 37%.

Northern Blotting Legume-Microbe Interactions Laboratory

Add 500 µl of phenol saturated with depc water pH 5.2 and vortex for 15. An RNA gel consists of 1.5% agarose in 1X MOPS buffer/16-17 % formaldehyde. 10X MOPS Buffer RNA Sample Buffer (per mL). Formamide, 562 uL. Formaldehyde (37%) Shake vigorously to get the DEPC into solution. Allow solution to. Buffers containing free amine groups (TRIS, HEPES, etc) and solutions/buffers that cannot be autoclaved (sucrose, MOPS, etc) cannot be DEPC-treated.